Identifying mismatch repair deficient colon cancer: near perfect concordance between immunohistochemistry and microsatellite instability testing in a large, population-based series

Identifying mismatch repair deficient colon cancer: near perfect concordance between immunohistochemistry and microsatellite instability testing in a large, population-based series

Objective: Establish a mismatch repair (MMR) status of colorectal cancer is essential to enable the detection of Lynch syndrome that underlie and inform prognosis and therapy. current testing usually involves either PCR-based microsatellite (MSI) testing or MMR protein immunohistochemistry (IHC). We aimed to compare the two approaches, a large population-based cohort of stage 2 and 3 cases of colon cancer in Northern Ireland.


Methods and results: Promega pentaplex Using this test, we determined the status of MSI’s four MMR IHC antibody panel. IHC applied to the tumor tissue microarray tumor samples in triplicate and scored manually. Of the 593 cases with available MSI and MMR IHC results, 136 (22.9%) were MSI-H and 135 (22.8%) displayed abnormal MMR IHC.

Concordance was very high, with 97.1% of MSI-H cases showed normal MMR IHC and 97.8% of cases with normal IHC showed MSI-H status. Under-representation of epithelial tumor cells in a sample of the tumor is very inflamed resulting in misclassification of some cases with microsatellite stable normal MMR IHC. MMR IHC revealed a rare case with unusual patterns of expression of MMR proteins, an unusual combination of loss of secondary clonal expression or loss of expression, is further illustrated by immunostaining repeated in all parts of the network.


Conclusions: PCR MSI and MMR IHC testing can be considered equally adept tests to establish the status of MMR / MSI, while aware of the potential pitfalls either method. Methodology selection may depend on the available services and expertise.


Anti-sclerostin monoclonal antibody romosozumab, treatment for osteoporosis, reduce the risk of vertebral fracture and clinical fracture. laser irradiation triggers a variety of effects, including bio-stimulation, which can induce beneficial effects of therapy and biological response. Initially, we performed in vivo experiments to clarify the mechanism better bone healing in bone laser ablated.

Here, we evaluated a comprehensive gene expression and sequence in the Er: YAG laser ablation, bur-drilled, and bones treated controls, and found the laser irradiation is pressed Sost (protein coding: sclerostin) expression in the bone, possibly through stimulation mechanotransducers. Surprisingly, the effect of laser bio-stimulation suppressed the expression of Sost in primary osteogenic cells.

Identifying mismatch repair deficient colon cancer: near perfect concordance between immunohistochemistry and microsatellite instability testing in a large, population-based series
Identifying mismatch repair deficient colon cancer: near perfect concordance between immunohistochemistry and microsatellite instability testing in a large, population-based series

SPARC is a key mediator of TGF-β-induced metastatic kidney cancer

Aberrant expression of transforming growth factor-β1 (TGF-β1) is associated with renal cell carcinoma (RCC) progression by inducing the cancer metastasis. However, downstream effector (s) in the TGF-β signaling pathway is not fully characterized. In this study, the height of secreted protein acidic and rich in cysteine ​​(SPARC) as regulated TGF-β genes in RCC were identified by applying the analysis of genes expressed differently and microarray analysis, we further confirmed these results in a few RCC cell lines.

Clinically, expression of both genes were positively correlated to the RCC patient specimens. Furthermore, increased expression of SPARC is found in all subtypes of RCC and RCC positively correlated with stage and grade. Instead, SPARC expression is inversely proportional to the overall and disease-free survival of patients with RCC, showing SPARC as a powerful prognostic marker of survival of RCC patients. SPARC knock down significantly inhibit invasion and metastasis of RCC cells both in vitro and in vivo.

p38

MBS8533658-01mLAF405S 0.1mL(AF405S)
EUR 565

p38

MBS8533658-01mLAF610 0.1mL(AF610)
EUR 565

p38

MBS8533658-01mLAF635 0.1mL(AF635)
EUR 565

P38 mAb

MBS435304-005mL 0.05mL
EUR 415

P38 mAb

MBS435304-01mL 0.1mL
EUR 660

P38 mAb

MBS435304-5x01mL 5x0.1mL
EUR 2655

p38 MAPK

E8ET1702-65 100ul
EUR 275
Description: Available in various conjugation types.

Mxi2, p38

MBS603655-01mg 0.1mg
EUR 820

Mxi2, p38

MBS603655-5x01mg 5x0.1mg
EUR 3530

p38 MAPK

MBS8534372-01mL 0.1mL
EUR 345

p38 MAPK

MBS8534372-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 MAPK

MBS8534372-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 MAPK

MBS8534372-01mLAF610 0.1mL(AF610)
EUR 565

p38 MAPK

MBS8534372-01mLAF635 0.1mL(AF635)
EUR 565

p38 protein

E20-80078 20ug
EUR 275
Description: N/A

p38 protein

E20-80079 20ug
EUR 275
Description: N/A

p38 protein

MBS8526800-002mg 0.02mg
EUR 345

p38 protein

MBS8526800-5x002mg 5x0.02mg
EUR 1410

p38 protein

MBS8526801-002mg 0.02mg
EUR 345

p38 protein

MBS8526801-5x002mg 5x0.02mg
EUR 1410

p38 Antibody

20-abx007541
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  • 30 ul
  • 100 ul
  • 200 ul

p38 antibody

70R-51505 100 ul
EUR 242
Description: Purified Polyclonal p38 antibody

p38 antibody

70R-51506 100 ul
EUR 286
Description: Purified Polyclonal p38 antibody

p38 Antibody

35478 100ul
EUR 479

p38 Antibody

35478-100ul 100ul
EUR 468

p38 Antibody

20-abx133223
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  • 30 ul
  • 100 ul
  • 200 ul

p38 Antibody

48644 100ul
EUR 499

p38 Antibody

48644-100ul 100ul
EUR 399.6

p38 Antibody

48644-50ul 50ul
EUR 286.8

p38 Antibody

20-abx141038
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p38 Antibody

B7178-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B7178-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0798-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0798-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0799-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0799-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

abx236076-100g 100 µg
EUR 350

p38 Antibody

abx236076-100ug 100 ug
EUR 577.2

p38 Antibody

E38PA4721 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

E38PA5060 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

E38PA5094 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

E38PA7126 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

E38PA3186 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

MBS5316121-01mL 0.1mL
EUR 460

p38 Antibody

MBS5316121-5x01mL 5x0.1mL
EUR 1920

p38 Antibody

MBS5316122-01mL 0.1mL
EUR 535

p38 Antibody

MBS5316122-5x01mL 5x0.1mL
EUR 2270

p38 Antibody

MBS8002013-0025mL 0.025mL
EUR 315

p38 Antibody

MBS8002013-5x0025mL 5x0.025mL
EUR 890

p38 Antibody

MBS805089-01mL 0.1mL
EUR 425

p38 Antibody

MBS805089-5x01mL 5x0.1mL
EUR 1350

p38 Antibody

MBS806203-0025mL 0.025mL
EUR 320

p38 Antibody

MBS8581787-01mL 0.1mL
EUR 305

p38 Antibody

MBS8581787-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8581787-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8581787-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8581787-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS8584173-01mL 0.1mL
EUR 305

p38 Antibody

MBS8584173-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8584173-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8584173-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8584173-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS8582665-01mL 0.1mL
EUR 305

p38 Antibody

MBS8582665-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8582665-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8582665-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8582665-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS8582905-01mL 0.1mL
EUR 305

p38 Antibody

MBS8582905-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8582905-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8582905-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8582905-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS8582923-01mL 0.1mL
EUR 305

p38 Antibody

MBS8582923-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8582923-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8582923-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8582923-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS9508977-005mL 0.05mL
EUR 285

p38 Antibody

MBS9508977-01mL 0.1mL
EUR 385

p38 Antibody

MBS9508977-5x01mL 5x0.1mL
EUR 1590

p38 Antibody

MBS9409774-01mL 0.1mL
EUR 420

p38 Antibody

MBS9409774-5x01mL 5x0.1mL
EUR 1740

p38 Antibody

MBS9435492-005mL 0.05mL
EUR 325

p38 Antibody

MBS9435492-01mL 0.1mL
EUR 435

p38 Antibody

MBS9435492-5x01mL 5x0.1mL
EUR 1810

p38 Antibody

SPC-172D 0.1ml
EUR 161
Description: Rabbit Anti-Human p38 Polyclonal

p38 Antibody

SPC-172S 0.012ml Ask for price
Description: Rabbit Anti-Human p38 Polyclonal

p38 Substrate

MBS517838-1mg 1mg
EUR 205

p38 Substrate

MBS517838-5x1mg 5x1mg
EUR 675

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS657552-10Blots 10Blots
EUR 430

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS657552-5x10Blots 5x10Blots
EUR 1785

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS633592-01mL 0.1mL
EUR 695

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS633592-5x01mL 5x0.1mL
EUR 2985

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS632290-02mL 0.2mL
EUR 660

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS632290-5x02mL 5x0.2mL
EUR 2810

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-02mL 0.2mL
EUR 650

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-06mL 0.6mL
EUR 1175

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-5x06mL 5x0.6mL
EUR 5140

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS627818-10Blots 10Blots
EUR 875

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS627818-5x10Blots 5x10Blots
EUR 3785

SB-203580 p38 MAPK(p38 MAP kinase) Inhibitor

E1B1863 1mg
EUR 85

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor

MBS8579825-1mg 1mg
EUR 180

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor

MBS8579825-5x1mg 5x1mg
EUR 660

p38 (411-425) M.leprae

MBS8246858-10mg 10mg
EUR 675

p38 (411-425) M.leprae

MBS8246858-1mg 1mg
EUR 255

p38 (411-425) M.leprae

MBS8246858-5mg 5mg
EUR 480

p38 (411-425) M.leprae

MBS8246858-5x10mg 5x10mg
EUR 2955

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)

MBS631660-04mL 0.4mL
EUR 670

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)

MBS631660-5x04mL 5x0.4mL
EUR 2870

Anti - p38-a

MBS684252-002mL 0.02mL
EUR 270

Anti - p38-a

MBS684252-005mL 0.05mL
EUR 485

Anti - p38-a

MBS684252-01mL 0.1mL
EUR 720

Anti - p38-a

MBS684252-5x01mL 5x0.1mL
EUR 2925

Anti - p38-b

MBS684253-002mL 0.02mL
EUR 270

Anti - p38-b

MBS684253-005mL 0.05mL
EUR 485

Anti - p38-b

MBS684253-01mL 0.1mL
EUR 720

Anti - p38-b

MBS684253-5x01mL 5x0.1mL
EUR 2925

Anti - p38-g

MBS684254-002mL 0.02mL
EUR 270

Anti - p38-g

MBS684254-005mL 0.05mL
EUR 485

Anti - p38-g

MBS684254-01mL 0.1mL
EUR 720

Anti - p38-g

MBS684254-5x01mL 5x0.1mL
EUR 2925

Anti - p38-d

MBS684255-002mL 0.02mL
EUR 270

Anti - p38-d

MBS684255-005mL 0.05mL
EUR 485

Anti - p38-d

MBS684255-01mL 0.1mL
EUR 720

Anti - p38-d

MBS684255-5x01mL 5x0.1mL
EUR 2925

p38 Mouse mAb

E2220879 100ul
EUR 225
Description: Available in various conjugation types.

p38 Mouse mAb

E2222580 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

p38 Mouse mAb

MBS8539086-01mL 0.1mL
EUR 305

p38 Mouse mAb

MBS8539086-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF610 0.1mL(AF610)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

E2340697 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb

E2380881 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb

E2381051 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb

E2R25239 100μl
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb

E2R27123 100μl
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb

E38A4061 100ug/100ul
EUR 255

p38 Rabbit pAb

E2251338 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb

E2251719 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb

MBS4754198-01mL 0.1mL
EUR 450

p38 Rabbit mAb

MBS4754198-5x01mL 5x0.1mL
EUR 1540

p38 Rabbit mAb

MBS4752931-01mL 0.1mL
EUR 450

p38 Rabbit mAb

MBS4752931-5x01mL 5x0.1mL
EUR 1540

p38 HOG Kinase

MBS395428-025mg 0.25mg
EUR 425

p38 HOG Kinase

MBS395428-5x025mg 5x0.25mg
EUR 1760

p38 Rabbit pAb

MBS8542346-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8542346-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8542346-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8542346-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8542346-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

MBS8542516-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8542516-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8542516-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8542516-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8542516-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

MBS8539985-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8539985-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8539985-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8539985-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8539985-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

MBS8541727-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8541727-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8541727-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8541727-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8541727-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

MBS8540327-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8540327-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8540327-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8540327-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8540327-01mLAF635 0.1mL(AF635)
EUR 565

anti-Crk p38

YF-PA23509 50 ul
EUR 400.8
Description: Mouse polyclonal to Crk p38

anti-Crk p38

YF-PA11088 50 ul
EUR 435.6
Description: Mouse polyclonal to Crk p38

anti-Crk p38

YF-PA11089 50 ug
EUR 435.6
Description: Mouse polyclonal to Crk p38

anti-Crk p38

YF-PA11090 100 ug
EUR 483.6
Description: Rabbit polyclonal to Crk p38

Similarly, in vitro cell invasion could be reduced by using a specific monoclonal antibody. Mechanically, SPARC activate protein kinase B (AKT) pathway which leads to an increase in the expression of matrix metalloproteinase-2, which can facilitate the invasion of RCC. Overall, our data support the SPARC is an important role of TGF-β signaling networks that underlie the development of RCC and potential therapeutic targets and prognostic markers.