Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants

Many infectious diseases that affect livestock impose significant economic burdens, and in some cases also a threat to humans and is classified as a zoonosis. Some zoonotic diseases transmitted by vectors and, due to environmental factors and socio-ecconomic complex, the distribution of many pathogens is changing, with a growing number of which are found in countries that were previously unaffected.

Here, we develop multiplex assays, microarray based suspension, is able to detect specific antibodies for the five important pathogen of cattle (three of them zoonoses) that currently appears in a new geographical location: Rift Valley fever virus (RVFV), Crimean- Congo hemorrhagic fever virus (CCHFV), Schmallenberg virus (SBV) Bluetongue virus (BTV) and the bacterium Mycobacterium complex tuberculosis.Using Luminex platform, polystyrene microspheres coated with recombinant protein from each of the five pathogens. The mixture of microspheres used for the simultaneous detection of antibodies against five diseases in accordance affecting ruminants.

The following pane sera included in this study: 50 sera from sheep experimentally infected with RVFV, 74 sera from calves and sheep vaccinated with SBV, 26 sera of cattle vaccinated with Mycobacterium bovis, 30 field sera from ruminant species differ infected CCHFV and 73 calf sera infected with BTV. Finally, to determine the diagnostic specificity of 220 field sera Spain frees her from the farm of five disease assessed. All sera were classified using a commercial ELISA specific to each disease, which is used in this study as a reference technique.

The results showed a multiplex test exhibited excellent performance characteristics with sensitivity ranging from 93-100% and specificity ranging 96-99% depending on the pathogen. This new tool enables the simultaneous detection of antibodies against five key pathogens, reducing the required sample volume and timing analysis where patogens is usually tested individually.

 Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants
Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants

optoelectrical highly sensitive biosensors for diagnosis allergic multiplex

Compact multiplexing biosensor system has great potential for the diagnosis of diseases in which the detection of multiple biomarkers is required. Immunoglobulin E-mediated hypersensitivity syndrome is a primary immunodeficiency disorders associated with sensitization to allergens. Assessing immunoglobulin E (IgE) sensitization to allergens is an important strategy for the diagnosis of allergies.

Here, we report for the first time a reliable, flexible optoelectrical biosensor system and cost-effective for the simultaneous determination of total and allergen-specific IgE and IgG antibodies using immunogold-silver method of signal amplification. This biosensor is built on the usual digital versatile disc (DVD) to immobilize a 12 panel of allergen extracts or purified protein in a microarray format, as a proof of concept.

The biosensor multiplexing indicates the limit of detection of 0.26 IU / mL (624 pg / mL) and 14 ng / mL for IgE and IgG antibodies, respectively. The system is successfully applied in a cohort of 127 human serum samples, showed good sensitivity (97.6%) and specificity (85.7%), and a very good area under the curve (AUC) values ​​found in 0.977 (confidence interval, CI 0.957 to 0.990) compared and validated with immunofluorescence assay clinical references, confirming the excellent correlation between the two techniques.

Frozen Tissue Section Panel - Mouse Normal Tissue, Multi-tissue I

T6334447 5 slides
EUR 504

Frozen Tissue Section Panel - Mouse Normal Tissue, Multi-tissue II

T6334608 5 slides
EUR 504

Frozen Tissue Section Panel - Mouse Normal Tissue, Multi-tissue III

T6334423 5 slides
EUR 504

Stomach carcinoma with matched stomach tissue microarray

ST2084b each
EUR 546
Description: Stomach carcinoma with matched stomach tissue microarray, containing 94 cases of adenocarcinoma, 1 each of signet ring cell carcinoma and squamous cell carcinoma, duplicated cores per case

EZ-TMA Manual Tissue Microarray Kit 3 - 3 mm x 24 Core

IW-123 -
EUR 125

EZ-TMA Manual Tissue Microarray Kit 4 - 4 mm x 15 Core

IW-124 -
EUR 125

Skin cancer and normal tissue high density (69 cases/208 core) tissue microarray

SK208 each
EUR 546
Description: Skin cancer and normal tissue high density (69 cases/208 core) tissue microarray

Frozen Tissue Section Panel - Rat Normal Tissue, Multi-tissue I

T6434448 5 slides
EUR 504

Frozen Tissue Section Panel - Rat Normal Tissue, Multi-tissue III

T6434423 5 slides
EUR 504

Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue I

T6234431 5 slides
EUR 507

Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue II

T6234432 5 slides
EUR 507

Frozen Tissue Section Panel - Human Adult Normal Tissue, Multi-tissue III

T6234433 5 slides
EUR 507

Frozen Tissue Section Panel - Monkey (Rhesus) Normal Tissue, Multi-tissue I

T6534448 5 slides
EUR 504

Frozen Tissue Section Panel - Monkey (Rhesus) Normal Tissue, Multi-tissue III

T6534423 5 slides
EUR 504

Frozen Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue I

T6534448-Cy 5 slides
EUR 504

Frozen Tissue Section Panel - Monkey (Cynomolgus) Normal Tissue, Multi-tissue III

T6534423-Cy 5 slides
EUR 504

Brain primary tumor high density (69 cases/208 cores) tissue microarray of astrocytoma

GL208 each
EUR 546
Description: Brain primary tumor high density (69 cases/208 cores) tissue microarray of astrocytoma, glioblastoma, glioblastoma multiforme (GBM) and normal tissue

EZpunch 1500 Manual Tissue Microarrayer

TMA-punch -
EUR 1850

AutoTiss 10C Automatic Tissue Microarrayer

AutoTiss10C -
EUR 98000

Quick-Ray Manual Tissue Microarrayer Full Set

IW-UT06 -
EUR 3800

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue I, 7 different tissues

T6236444Alz 5 slides
EUR 1135

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue II, 7 different tissues

T6236445Alz 5 slides
EUR 1135

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue III, 8 different tissues

T6236446Alz 5 slides
EUR 1135

Frozen Tissue Section Panel - Human Disease Tissue, Alzheimer's Disease, Multi-tissue IV, 7 different tissues

T6236564Alz 5 slides
EUR 1135

AutoTiss One Semi-Automatic Tissue Microarrayer

TMA-001 -
EUR 25800

Microarray Stripping Buffer 1X

10250005-1 25 mL
EUR 21.5

Microarray Stripping Buffer 1X

10250005-2 100 mL
EUR 60.54

Quick-Ray Master Automated Tissue Microarrayer UATM-272B

UATM-272B -
EUR 98000

Frozen Tissue Pen

25469-1 1each
EUR 195

384 Well Storage Microarray Plates, V-Shaped Wells

GP712 50 Plates
EUR 602.4

384 Well Storage Microarray Plates, Cylindrical Wells

GP711 50 Plates
EUR 602.4

Frozen Tissue Section - Lupus: Pancreas

T1236188Lup 5 slides
EUR 515

FDA Standard Frozen Tissue Array - Mouse Normal

T6334701-1 2 slides
EUR 629

FDA Standard Frozen Tissue Array - Mouse Normal

T6334701-2 4 slides
EUR 1135

Frozen Tissue Section - Human Diabetic Diseased Tissue: Pancreas

T1236188Dia 5 slides
EUR 475

Frozen Tissue Section - Human Tumor: Pancreas

T1235188 5 slides
EUR 330

Rabbit MUSCLE TISSUE MT FROZEN 1kg

41325-2 1 kg
EUR 337.69

Frozen Tissue Section - Asthma: Lung

T1236152Ld-1 5 slides
EUR 475

Frozen Tissue Section - Lupus: Ovary

T1236183Lup 5 slides
EUR 682

Frozen Tissue Section - Lupus: Kidney

T1236142Lup 5 slides
EUR 587

Frozen Tissue Section - Lupus: Spleen

T1236246Lup 5 slides
EUR 515

Frozen Tissue Section - Lupus: Adrenal

T1236004Lup 5 slides
EUR 515

Frozen Tissue Section - Emphysema: Lung

T1236152Ld-3 5 slides
EUR 475

Frozen Tissue Section - Pneumonia: Lung

T1236152Ld-4 5 slides
EUR 475

Frozen Tissue Section - Bronchitis: Lung

T1236152Ld-2 5 slides
EUR 475

Frozen Tissue Section - Human Adult Normal: Pancreas

T1234188 5 slides
EUR 343

Frozen Tissue Section - hypertension: Vein

T1236020Hd-2 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Eye

T1236108Dia 5 slides
EUR 662

Frozen Tissue Section - hypertension: Heart

T1236122Hd-2 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Lung

T1236152Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Skin

T1236218Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Brain

T1236035Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Colon

T1236090Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Heart

T1236122Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Liver

T1236149Dia 5 slides
EUR 475

Frozen Tissue Section - hypertension: Artery

T1236013Hd-2 5 slides
EUR 475

Frozen Tissue Section - hypertension: Kidney

T1236142Hd-2 5 slides
EUR 475

Frozen Tissue Array - Human Adult Normal Tissue, Multi-tissue I

T6234700-1 1 slide
EUR 265

Frozen Tissue Array - Human Adult Normal Tissue, Multi-tissue I

T6234700-5 5 slides
EUR 1004

Frozen Tissue Section - Human Tumor: Bone

T1235023 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Lung

T1235152 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Skin

T1235218 5 slides
EUR 330

Frozen Tissue Section - Human Diabetic Diseased Tissue: Kidney

T1236142Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Spleen

T1236246Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Adrenal

T1236004Dia 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Stomach

T1236248Dia 5 slides
EUR 475

Frozen Tissue Section - Human Tumor: Brain

T1235035 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Colon

T1235090 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Liver

T1235149 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Ovary

T1235183 5 slides
EUR 330

Frozen Tissue Section - Lupus: Spinal Cord

T1236234Lup 5 slides
EUR 515

Frozen Tissue Section - Human Tumor: Breast

T1235086 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Kidney

T1235142 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Rectum

T1235206 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Testis

T1235260 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Uterus

T1235274 5 slides
EUR 330

Frozen Tissue Section - Arteriosclerosis: Aorta

T1236012Hd-4 5 slides
EUR 1060

Frozen Tissue Section - Human Diabetic Diseased Tissue: Esophagus

T1236106Dia 5 slides
EUR 475

Frozen Tissue Section - Human Tumor: Adrenal

T1235004 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Bladder

T1235010 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Parotid

T1235190 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Stomach

T1235248 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Thymoma

T1235264 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Thyroid

T1235265 5 slides
EUR 330

Frozen Tissue Section - Arteriosclerosis: Artery

T1236013Hd-4 5 slides
EUR 1060

Frozen Tissue Array - Dog Male Adult Normal Tissue, Multi-tissue I

T6734700-G01-1 1 slide
EUR 239

Frozen Tissue Array - Dog Male Adult Normal Tissue, Multi-tissue I

T6734700-G01-5 5 slides
EUR 883

Frozen Tissue Section - Human Tumor: Lymphoma

T1235161 5 slides
EUR 330

Frozen Tissue Section - Human Tumor: Prostate

T1235201 5 slides
EUR 330

Multiple Species Frozen Tissue Array - Brain

T6134035-1 1 slide
EUR 195

Multiple Species Frozen Tissue Array - Brain

T6134035-5 5 slides
EUR 711

Multiple Species Frozen Tissue Array - Liver

T6134149-1 1 slide
EUR 195

Multiple Species Frozen Tissue Array - Liver

T6134149-5 5 slides
EUR 711

Frozen Tissue Array - Human Tumor and Normal Tissue, Multi-tissue I

T6235700-1 1 slide
EUR 415

Frozen Tissue Array - Human Tumor and Normal Tissue, Multi-tissue I

T6235700-5 5 slides
EUR 1280

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I

T6734700-G02-1 1 slide
EUR 239

Frozen Tissue Array - Dog Female Adult Normal Tissue, Multi-tissue I

T6734700-G02-5 5 slides
EUR 883

Frozen Tissue Section - Human Tumor: Esophagus

T1235106 5 slides
EUR 330

Frozen Tissue Array - Rabbit Male Adult Normal Tissue, Multi-tissue I

T6834700-G01-1 1 slide
EUR 239

Frozen Tissue Array - Rabbit Male Adult Normal Tissue, Multi-tissue I

T6834700-G01-5 5 slides
EUR 883

Frozen Tissue Section - Human Diabetic Diseased Tissue: Bone Marrow

T1236024Dia 5 slides
EUR 662

Frozen Tissue Section - Alzheimer's Disease: Brain

T1236035Alz 5 slides
EUR 475

Frozen Tissue Section - Parkinson's Disease: Brain

T1236035Par 5 slides
EUR 475

Frozen Tissue Section - Dementia: Brain: Thalamus

T1236079Dem 5 slides
EUR 475

Frozen Tissue Section - Lupus: Heart: Pericardium

T1236133Lup 5 slides
EUR 515

Frozen Tissue Section - Pulmonary embolism: Lung

T1236152Ld-5 5 slides
EUR 475

Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I

T6834700-G02-1 1 slide
EUR 239

Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I

T6834700-G02-5 5 slides
EUR 883

FDA Standard Frozen Tissue Array - Rat Normal

T6434701-1 2 slides
EUR 629

FDA Standard Frozen Tissue Array - Rat Normal

T6434701-2 4 slides
EUR 1135

Frozen Tissue Section - Arrhythmia, infarct: Heart

T1236122Hd-1 5 slides
EUR 475

Frozen Tissue Section - Liver Cirrhosis: Prostate

T1236201Lcs 5 slides
EUR 475

Frozen Tissue Section - Depression: Brain: Thalamus

T1236079Dep 5 slides
EUR 475

Frozen Tissue Section - Liver Cirrhosis: Esophagus

T1236106Lcs 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Visceral Adipose

T1236003VisDia 5 slides
EUR 662

Frozen Tissue Section - Human Diabetic Diseased Tissue: Skeletal Muscle

T1236171Dia 5 slides
EUR 475

Frozen Tissue Section - Human Adult Normal: Lung

T1234152 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Skin

T1234218 5 slides
EUR 343

Frozen Tissue Section - Dementia: Brain: Hippocampus

T1236052Dem 5 slides
EUR 883

Paraffin Tissue Section Panel - Mouse Whole Brain Segmentation Panel

T8334035 5 slides
EUR 580

Frozen Tissue Section - Human Adult Normal: Brain

T1234035 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Colon

T1234090 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Heart

T1234122 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Liver

T1234149 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Ovary

T1234183 5 slides
EUR 629

Frozen Tissue Section - Human Adult Normal: Penis

T1234194 5 slides
EUR 343

Frozen Tissue Section - Depression: Brain: Hippocampus

T1236052Dep 5 slides
EUR 883

Frozen Tissue Section - Human Adult Normal: Breast

T1234086 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Retina

T1234110 5 slides
EUR 529

Frozen Tissue Section - Human Adult Normal: Kidney

T1234142 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Rectum

T1234206 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Spleen

T1234246 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Testis

T1234260 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Throat

T1234263 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Uterus

T1234274 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Vagina

T1234283 5 slides
EUR 343

Frozen Tissue Section - Lupus: Blood Vessel: Artery

T1236013Lup 5 slides
EUR 515

Frozen Tissue Section - Human Diabetic Diseased Tissue: Subcutaneous Adipose

T1236003SubDia 5 slides
EUR 662

Frozen Tissue Section - Dementia: Brain: Frontal Lobe

T1236051Dem 5 slides
EUR 475

Frozen Tissue Section - Human Adult Normal: Adipose

T1234003 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Adrenal

T1234004 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Bladder

T1234010 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Trachea

T1234160 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Parotid

T1234190 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Stomach

T1234248 5 slides
EUR 343

TMB for Microarrays

42R-1004 500 ml
EUR 424.8
Description: Ultra sensitive TMB Peroxidase Substrate ready to use in immunoarrays.

Frozen Tissue Section - Human Adult Normal: Appendix

T1234006 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Placenta

T1234200 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Prostate

T1234201 5 slides
EUR 343

Frozen Tissue Section - Depression: Brain: Frontal Lobe

T1236051Dep 5 slides
EUR 475

Frozen Tissue Section - Human Adult Normal: Esophagus

T1234106 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Diaphragm

T1234169 5 slides
EUR 343

Frozen Tissue Section - Dementia: Brain: Parietal Lobe

T1236066Dem 5 slides
EUR 475

Frozen Tissue Section - Alzheimer's Disease: Brain: Pons

T1236071Alz 5 slides
EUR 475

Frozen Tissue Section - Parkinson's Disease: Brain: Pons

T1236071Par 5 slides
EUR 475

Frozen Tissue Section - Dementia: Brain: Temporal Lobe

T1236078Dem 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Small Intestine: Ileum

T1236227Dia 5 slides
EUR 475

Frozen Tissue Section - Human Adult Normal: Epididymis

T1234105 5 slides
EUR 343

Frozen Tissue Section - Dementia: Brain: Occipital Lobe

T1236062Dem 5 slides
EUR 475

Paraffin Tissue Section Panel - Mouse Normal Tissue, Multi-tissue I

T8334447 5 slides
EUR 242

Paraffin Tissue Section Panel - Mouse Normal Tissue, Multi-tissue II

T8334608 5 slides
EUR 242

Frozen Tissue Section - Human Diabetic Diseased Tissue: Small Intestine: Jejunum

T1236230Dia 5 slides
EUR 475

Paraffin Tissue Section Panel - Mouse Normal Tissue, Multi-tissue III

T8334423 5 slides
EUR 242

Frozen Tissue Section - Human Adult Normal: Gallbladder

T1234118 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Parathyroid

T1234189 5 slides
EUR 758

Frozen Tissue Section - Depression: Brain: Parietal Lobe

T1236066Dep 5 slides
EUR 475

Frozen Tissue Section - Depression: Brain: Temporal Lobe

T1236078Dep 5 slides
EUR 475

Frozen Tissue Section - Congenital heart disease: Heart

T1236122Hd-3 5 slides
EUR 475

Frozen Tissue Section - Congestive Heart Failure: Heart

T1236122Hd-6 5 slides
EUR 794

Frozen Tissue Section - hypertension: Heart Atrium, left

T1236126Hd-2 5 slides
EUR 475

Frozen Tissue Section - Depression: Brain: Occipital Lobe

T1236062Dep 5 slides
EUR 475

Frozen Tissue Section - hypertension: Heart Atrium, right

T1236127Hd-2 5 slides
EUR 475

Frozen Tissue Section - Human Diabetic Diseased Tissue: Small Intestine: Duodenum

T1236101Dia 5 slides
EUR 475

Frozen Tissue Section - Human Adult Normal: Brain: Pons

T1234071 5 slides
EUR 343

Frozen Tissue Section - Human Adult Normal: Lymph Node

T1234161 5 slides
EUR 343

FDA Standard Frozen Tissue Array - Human Adult Normal

T6234701-1 2 slides
EUR 1225

Multiplex biosensor system with on-demand panel composition can be used completely autonomously in a clinical laboratory setting or mobile without the need for additional medical equipment, with which could make it suitable for large allergy screening campaigns to better define the profile sensitization.