Category: PCR

August 16, 2020

Antibody microarray analysis of serum inflammatory cytokines in patients with calcific aortic valve disease

Background: Calcific aortic valve disease (CAVD) is a slowly progressive pathological process associated with significant morbidity and mortality, CAVD is heart valve disease most common in older people and is a major cause of aortic valve stenosis. Some steps characterize the process: inflammation, apoptosis, lipid deposition, activation of the renin-angiotensin system, extracellular matrix remodeling, and bone formation. This paper focuses on detecting and analyzing the […]

August 16, 2020

The HIF target ATG9A is Essential for Epithelial Barrier Function and Tight Junction Biogenesis

intestinal epithelial cells (IECs) present in the metabolic state of low oxygen tension is called “physiological hypoxia.” An important factor in maintaining intestinal homeostasis is a transcription factor hypoxia-inducible factor (HIF), which is stabilized under hypoxic conditions and mediate the homeostatic response to low oxygen tension IEC. To identify the target HIF transcription in IEC, chromatin immunoprecipitation (CHIP) is performed in Caco-2 IECs using HIF-1α […]

August 16, 2020

Development of a multiplex assay for antibody detection in serum against pathogens affecting ruminants

Many infectious diseases that affect livestock impose significant economic burdens, and in some cases also a threat to humans and is classified as a zoonosis. Some zoonotic diseases transmitted by vectors and, due to environmental factors and socio-ecconomic complex, the distribution of many pathogens is changing, with a growing number of which are found in countries that were previously unaffected. Here, we develop multiplex assays, […]