The HIF target ATG9A is Essential for Epithelial Barrier Function and Tight Junction Biogenesis

intestinal epithelial cells (IECs) present in the metabolic state of low oxygen tension is called “physiological hypoxia.” An important factor in maintaining intestinal homeostasis is a transcription factor hypoxia-inducible factor (HIF), which is stabilized under hypoxic conditions and mediate the homeostatic response to low oxygen tension IEC.

To identify the target HIF transcription in IEC, chromatin immunoprecipitation (CHIP) is performed in Caco-2 IECs using HIF-1α or HIF-2α specific antibodies. CHIP-enriched DNA is hybridized to a promoter microarray custom (called CHIP-chip). Approach identified as containing autophagy as HIF-1 pathway in IEC main target. The binding of HIF-1 promoter ATG9A, only the transmembrane component in autophagy pathway, specifically enriched by exposure of IEC hypoxia.

This validation CHIP-chip revealed induction of ATG9A prominent, and tests promoter luciferase identified hypoxia response element functionally upstream of the TSS. hypoxia-mediated induction ATG9A lost in cells lacking HIF-1. Strikingly, we found that lentiviral-mediated knockdown ATG9A in IECs prevent epithelial barrier formation by> 95% and result in significant mislocalization of some tight junction (TJ) proteins.

Extensions of these findings suggest that ATG9A knockdown cells had intrinsic defects in the actin cytoskeleton, including mislocalization of TJ protein binding vasodilator-stimulated phosphoprotein (VASP). These results involve ATG9A as important to a few steps from the epithelial TJ biogenesis and actin cytoskeletal regulation. Our findings have a new application for a disorder involving epithelial barrier is compromised and suggest that targeting ATG9A may be a rational strategy for future therapeutic intervention.


paper reported one-step point-of-care testing (POCT) quantitative and sensitive chip built on the substrate patterned with microspots polydopamine perfluorinated arrays. Catching antibodies covalently move in microspots polydopamine, while the fluorescently labeled detection antibody is physically adsorbed on the surface of perfluorinated.

Chip POCT allowed one-step sandwich immunoassay and is able to directly detect analytes of whole blood without preprocessing sample. By taking further advantage of strong cooling fluorescence capabilities of polydopamine the free blocking substrate capable of achieving the same performance in detecting and measuring protein biomarker as a substrate with blocking treatment.

 The HIF target ATG9A is Essential for Epithelial Barrier Function and Tight Junction Biogenesis
The HIF target ATG9A is Essential for Epithelial Barrier Function and Tight Junction Biogenesis

A new scoring system immunohistochemistry revealed the association of the C-terminal MET, ectodomain shedding and loss of E-cadherin with poor prognosis in oral squamous cell carcinoma

Using a tissue microarray showed that the C-terminal membrane immunoreactivity and MET ectodomain (ECD) shedding associated with poor prognosis in cancer of the mouth. Visible potential diagnostic value, extrapolation of these results to all parts of the network investigated.

Since MET orchestrates the epithelial to mesenchymal transition (EMT), the result refers to the loss of E-cadherin; readings for EMT known to be associated with a poor prognosis. MET C-terminal, N-terminal MET, and E-cadherin immunoreactivities checked on paraffin-embedded formalin-fixed sections 203 parallel oral cancer using the antibody clones D1C2, A2H2-3 and NCH-38.

p38

MBS8533658-01mLAF405S 0.1mL(AF405S)
EUR 565

p38

MBS8533658-01mLAF610 0.1mL(AF610)
EUR 565

p38

MBS8533658-01mLAF635 0.1mL(AF635)
EUR 565

P38 mAb

MBS435304-005mL 0.05mL
EUR 415

P38 mAb

MBS435304-01mL 0.1mL
EUR 660

P38 mAb

MBS435304-5x01mL 5x0.1mL
EUR 2655

p38 MAPK

E8ET1702-65 100ul
EUR 275
Description: Available in various conjugation types.

Mxi2, p38

MBS603655-01mg 0.1mg
EUR 820

Mxi2, p38

MBS603655-5x01mg 5x0.1mg
EUR 3530

p38 MAPK

MBS8534372-01mL 0.1mL
EUR 345

p38 MAPK

MBS8534372-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 MAPK

MBS8534372-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 MAPK

MBS8534372-01mLAF610 0.1mL(AF610)
EUR 565

p38 MAPK

MBS8534372-01mLAF635 0.1mL(AF635)
EUR 565

p38 protein

E20-80078 20ug
EUR 275
Description: N/A

p38 protein

E20-80079 20ug
EUR 275
Description: N/A

p38 protein

MBS8526800-002mg 0.02mg
EUR 345

p38 protein

MBS8526800-5x002mg 5x0.02mg
EUR 1410

p38 protein

MBS8526801-002mg 0.02mg
EUR 345

p38 protein

MBS8526801-5x002mg 5x0.02mg
EUR 1410

p38 Antibody

20-abx141038
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  • 100 ul
  • 200 ul
  • 30 ul

p38 Antibody

20-abx133223
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  • 100 ul
  • 200 ul
  • 30 ul

p38 Antibody

20-abx007541
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  • 100 ul
  • 200 ul
  • 30 ul

p38 Antibody

48644 100ul
EUR 499

p38 Antibody

48644-100ul 100ul
EUR 399.6

p38 Antibody

48644-50ul 50ul
EUR 286.8

p38 Antibody

35478 100ul
EUR 479

p38 Antibody

35478-100ul 100ul
EUR 468

p38 Antibody

abx236076-100ug 100 ug
EUR 577.2

p38 Antibody

E38PA4721 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

E38PA5060 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

E38PA5094 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

E38PA7126 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody

E38PA3186 100ul
EUR 225
Description: Available in various conjugation types.

p38 antibody

70R-51505 100 ul
EUR 242
Description: Purified Polyclonal p38 antibody

p38 antibody

70R-51506 100 ul
EUR 286
Description: Purified Polyclonal p38 antibody

p38 Antibody

abx236076-100g 100 µg
EUR 350

p38 Antibody

MBS9409774-01mL 0.1mL
EUR 420

p38 Antibody

MBS9409774-5x01mL 5x0.1mL
EUR 1740

p38 Antibody

MBS806203-0025mL 0.025mL
EUR 320

p38 Antibody

MBS8002013-0025mL 0.025mL
EUR 315

p38 Antibody

MBS8002013-5x0025mL 5x0.025mL
EUR 890

p38 Antibody

MBS805089-01mL 0.1mL
EUR 425

p38 Antibody

MBS805089-5x01mL 5x0.1mL
EUR 1350

p38 Antibody

MBS8584173-01mL 0.1mL
EUR 305

p38 Antibody

MBS8584173-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8584173-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8584173-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8584173-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS8582665-01mL 0.1mL
EUR 305

p38 Antibody

MBS8582665-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8582665-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8582665-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8582665-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS8582905-01mL 0.1mL
EUR 305

p38 Antibody

MBS8582905-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8582905-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8582905-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8582905-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS8582923-01mL 0.1mL
EUR 305

p38 Antibody

MBS8582923-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8582923-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8582923-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8582923-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS8581787-01mL 0.1mL
EUR 305

p38 Antibody

MBS8581787-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody

MBS8581787-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody

MBS8581787-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody

MBS8581787-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody

MBS5316121-01mL 0.1mL
EUR 460

p38 Antibody

MBS5316121-5x01mL 5x0.1mL
EUR 1920

p38 Antibody

MBS5316122-01mL 0.1mL
EUR 535

p38 Antibody

MBS5316122-5x01mL 5x0.1mL
EUR 2270

p38 Antibody

MBS9435492-005mL 0.05mL
EUR 325

p38 Antibody

MBS9435492-01mL 0.1mL
EUR 435

p38 Antibody

MBS9435492-5x01mL 5x0.1mL
EUR 1810

p38 Antibody

MBS9508977-005mL 0.05mL
EUR 285

p38 Antibody

MBS9508977-01mL 0.1mL
EUR 385

p38 Antibody

MBS9508977-5x01mL 5x0.1mL
EUR 1590

p38 Antibody

B0798-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0798-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0799-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B0799-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B7178-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody

B7178-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Substrate

MBS517838-1mg 1mg
EUR 205

p38 Substrate

MBS517838-5x1mg 5x1mg
EUR 675

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS657552-10Blots 10Blots
EUR 430

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS657552-5x10Blots 5x10Blots
EUR 1785

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-02mL 0.2mL
EUR 650

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-06mL 0.6mL
EUR 1175

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS630803-5x06mL 5x0.6mL
EUR 5140

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS632290-02mL 0.2mL
EUR 660

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS632290-5x02mL 5x0.2mL
EUR 2810

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS633592-01mL 0.1mL
EUR 695

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS633592-5x01mL 5x0.1mL
EUR 2985

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS627818-10Blots 10Blots
EUR 875

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)

MBS627818-5x10Blots 5x10Blots
EUR 3785

SB-203580 p38 MAPK(p38 MAP kinase) Inhibitor

E1B1863 1mg
EUR 85

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor

MBS8579825-1mg 1mg
EUR 180

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor

MBS8579825-5x1mg 5x1mg
EUR 660

p38 (411-425) M.leprae

MBS8246858-10mg 10mg
EUR 675

p38 (411-425) M.leprae

MBS8246858-1mg 1mg
EUR 255

p38 (411-425) M.leprae

MBS8246858-5mg 5mg
EUR 480

p38 (411-425) M.leprae

MBS8246858-5x10mg 5x10mg
EUR 2955

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)

MBS631660-04mL 0.4mL
EUR 670

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)

MBS631660-5x04mL 5x0.4mL
EUR 2870

Anti - p38-a

MBS684252-002mL 0.02mL
EUR 270

Anti - p38-a

MBS684252-005mL 0.05mL
EUR 485

Anti - p38-a

MBS684252-01mL 0.1mL
EUR 720

Anti - p38-a

MBS684252-5x01mL 5x0.1mL
EUR 2925

Anti - p38-b

MBS684253-002mL 0.02mL
EUR 270

Anti - p38-b

MBS684253-005mL 0.05mL
EUR 485

Anti - p38-b

MBS684253-01mL 0.1mL
EUR 720

Anti - p38-b

MBS684253-5x01mL 5x0.1mL
EUR 2925

Anti - p38-g

MBS684254-002mL 0.02mL
EUR 270

Anti - p38-g

MBS684254-005mL 0.05mL
EUR 485

Anti - p38-g

MBS684254-01mL 0.1mL
EUR 720

Anti - p38-g

MBS684254-5x01mL 5x0.1mL
EUR 2925

Anti - p38-d

MBS684255-002mL 0.02mL
EUR 270

Anti - p38-d

MBS684255-005mL 0.05mL
EUR 485

Anti - p38-d

MBS684255-01mL 0.1mL
EUR 720

Anti - p38-d

MBS684255-5x01mL 5x0.1mL
EUR 2925

p38 Mouse mAb

E2220879 100ul
EUR 225
Description: Available in various conjugation types.

p38 Mouse mAb

E2222580 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

p38 Mouse mAb

MBS8539086-01mL 0.1mL
EUR 305

p38 Mouse mAb

MBS8539086-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF610 0.1mL(AF610)
EUR 565

p38 Mouse mAb

MBS8539086-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

E2340697 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb

E2380881 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb

E2381051 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb

E2R25239 100μl
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb

E2R27123 100μl
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb

E2251338 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb

E2251719 100ul
EUR 225
Description: Available in various conjugation types.

anti-Crk p38

YF-PA11088 50 ul
EUR 435.6
Description: Mouse polyclonal to Crk p38

anti-Crk p38

YF-PA11089 50 ug
EUR 435.6
Description: Mouse polyclonal to Crk p38

anti-Crk p38

YF-PA11090 100 ug
EUR 483.6
Description: Rabbit polyclonal to Crk p38

anti-Crk p38

YF-PA23509 50 ul
EUR 400.8
Description: Mouse polyclonal to Crk p38

p38 Rabbit pAb

MBS8539985-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8539985-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8539985-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8539985-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8539985-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

MBS8540327-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8540327-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8540327-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8540327-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8540327-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

MBS8541727-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8541727-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8541727-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8541727-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8541727-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

MBS8542346-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8542346-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8542346-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8542346-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8542346-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb

MBS8542516-01mL 0.1mL
EUR 305

p38 Rabbit pAb

MBS8542516-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb

MBS8542516-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb

MBS8542516-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb

MBS8542516-01mLAF635 0.1mL(AF635)
EUR 565

p38 HOG Kinase

MBS395428-025mg 0.25mg
EUR 425

p38 HOG Kinase

MBS395428-5x025mg 5x0.25mg
EUR 1760

p38 Rabbit mAb

MBS4752931-01mL 0.1mL
EUR 450

p38 Rabbit mAb

MBS4752931-5x01mL 5x0.1mL
EUR 1540

p38 Rabbit mAb

MBS4754198-01mL 0.1mL
EUR 450

p38 Rabbit mAb

MBS4754198-5x01mL 5x0.1mL
EUR 1540

p38 beta, Active

7763-100 each
EUR 5214

p38 beta, Active

7763-5 each
EUR 444

p38 beta protein

30R-2875 5 ug
EUR 425
Description: Purified recombinant Human p38 beta protein

Crk p38 Antibody

AF6323 200ul
EUR 420

Crk p38 Antibody

AF6323-100ul 100ul
EUR 280

Crk p38 Antibody

AF6323-200ul 200ul
EUR 350

Inter- and intra-antibody relationship examined using the new scoring system, the distribution and the nonparametric median test. used survival analysis to examine the prognostic value immunoreactivities observed. The assessment of the three clones express the MET protein status (no, the feed, the C-terminal transmembrane positive), ECD shedding and EMT.