DNA Microarray Protocols.
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| A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis - http://www.tigr.org/tdb/microarray/conciseguide.html Hegde et al., Array Fabrication, microarray Printing, Probe Preparation and Hybridization, RNA Extraction, RNA labeling, Data Collection, Normalization, and Analysis.Institute for Genomic Research, Rockville, MD - [Read more] |
| A Concise Guide to cDNA Microarray Analysis ? II PDF. - http://pga.tigr.org/PDF/BiotechniquesCookbook_II.pdf Micro Array Fabrication, Probe Preparation and Hybridization, and Data Collection, Normalization and Analysis. Hegde, et al. biotechniques 2000. - [Read more] |
| Amino-allyl Labeling - http://pga.tigr.org/sop/M004_1a.pdf This protocol describes the labeling of eukaryotic RNA with aminoallyl labeled nucleotides via first strand cDNA synthesis followed by a coupling of the aminoallyl groups to either Cyanine 3 or 5 (Cy 3/Cy5) fluorescent molecules. Hasseman. TIGR Microarr - [Read more] |
| cDNA Synthesis and Labeling for Microarray Protocol - http://www.transcriptome.ens.fr/sgdb/protocols/labelling.php cDNA Synthesis and Labeling for Microarray Protocol. Mouse cDNA Synthesis and Labelling protocols. Yeast cDNA Synthesis and Labelling protocols. ENS transcriptome platform web site, Ile de France Genopole. - [Read more] |
| Chromatin Immunoprecipitation Protocol for Microarray Analysis - http://www.microarrays.ca/support/PDFs/ChIP_Protocol_for_Microarray_Analysis_Beads_09-07-04.pdf Chromatin Immunoprecipitation Protocol for Microarray Analysis ? Protein A/G Bead Method. Protocol From the UHN Microarray Centre - [Read more] |
| Clustering Tutorial - http://www.transcriptome.ens.fr/sgdb/tools/clustering.php Clustering Tutorial. ENS. - [Read more] |
| Complete Microarray Chip Protocol - http://www.rbhrfcrc.qimr.edu.au/kidney/Pages/Microarray%20protocol.html Reagents & Equipment, Labelling, Purification, Hybridisation, Washing. KRL Kidney Research Dr.Healt - [Read more] |
| Cyanine Dye Purification Protocol Cy - http://cmgm.stanford.edu/pbrown/mguide/hplc.html Cyanine Dye Purification Protocol using Sep-Pak and HPLC purification protocol for Cy dyes (un)incorporated with dNTP. Brown Lab Howard Hughes Medical Institute - [Read more] |
| DNA Microarray Procedures - http://omrf.ouhsc.edu/~frank/marraytraining.html Protocols, Frequently Asked Questions, Validation. Dr. Bart Frank - [Read more] |
| DNA microarray results normalization Tutorial - http://www.transcriptome.ens.fr/sgdb/tools/normalization.php DNA microarray results normalization Tutorial. ENS. - [Read more] |
| DNA Microarray Slide Production - http://www.transcriptome.ens.fr/sgdb/protocols/production.php DNA Microarray Slide Production for Microarrays. SYBR green labelling protocol used for DNA microarray quality control, SYBR green quality controls. ENS transcriptome platform web site, Ile de France Genopole - [Read more] |
| Fabrication Protocol for DNA Microarrays - http://research.nhgri.nih.gov/microarray/fabrication.shtml This protocol describes the steps required to produce a cDNA microarray. Gene-specific DNA is produced by PCR amplification of purified template plasmid DNAs from cloned ESTs. The PCR product is purified by ethanol precipitation, thoroughly resuspended in - [Read more] |
| Human DNA Microarray Hybridization - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_human.html Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation Washing and Scanning Arrays. Brown Lab. - [Read more] |
| Hybridization of Arrays - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_yeast.html Hybridization of Arrays. . General/Yeast Microarrays. Brown Lab - [Read more] |
| Hybridization Protocol for Hybridizing fluorescent cDNA to slide - http://research.nhgri.nih.gov/microarray/hybridization.shtml Hybridization Protocol for Hybridizing fluorescent cDNA to slide. - [Read more] |
| Image Analysis Microarray Tutorial - http://www.transcriptome.ens.fr/sgdb/tools/image_analysis.php Image Analysis Microarray Tutorial. Software used on the transcriptome platform, Software start-up, introduction to the software interface, Image acquisition, different steps of a microarray image analysis, Useful links. ENS transcriptome platform web site Ile de France Genopole - [Read more] |
| Labeled Probe Hybridization Procedure - http://pga.tigr.org/sop/M005_1a.pdf This protocol describes the hybridization of a Cy labeled cDNA probe (mix of Cy3 and Cy5) onto coated slide spotted with PCR amplified cDNA. TIGR Microarray Protocols. - [Read more] |
| List of DNA Microarray Protocols - http://derisilab.ucsf.edu/microarray/protocols.html List of DNA Microarray Protocols. microarrays.org - [Read more] |
| Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate - http://www.oup.co.uk/pdf/pas/6-5-3.pdf Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate. Elder et al., Oxford Practical Approach Series. - [Read more] |
| MAKING MICROARRAY PRINTING PLATES (IN DMSO) - http://pga.tigr.org/sop/M003_1a.pdf This protocol describes the method for making microarray printing plates in a 96 well format. (The same procedure applies to a 384 well format as well.) Hasseman. TIGR Microarray Protocols - [Read more] |
| Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorec - http://nar.oxfordjournals.org/cgi/content/full/33/5/e46 Kimura et al., NAR 2005. "easy-handling technology provided reproducible and precise measurement of methylated CpGs in MGMT promoter and, thus, our method may bring about a potential evolution in the handling of a variety of high-throughput DNA methylatio - [Read more] |
| MICROARRAY cDNA CLONE GROWTH AND TEMPLATE - http://pga.tigr.org/sop/M001_1a.pdf This protocol describes clone handling, plate replication, and DNA template preparation in a 96 well format. Hasseman. TIGR Microarray Protocols - [Read more] |
| MICROARRAY PCR, PURIFICATION, AND STORAGE - http://pga.tigr.org/sop/M002_1a.pdf This protocol describes PCR amplification of eukaryotic cDNA plasmid inserts, gel electrophoresis, purification, and storage of PCR products. Hasseman. TIGR Microarray Protocols. - [Read more] |
| Microarray Protocols - http://www.etox.ucr.edu/gill/Microarray_Protocols.html Heather R. Sanders. Hybridization, Probe Creation and RNA preparation for Microarray Protocols. - [Read more] |
| Microarray troubleshooting: image gallery - http://stress-genomics.org/stress.fls/expression/array_tech/trouble_shooting/troubles_index.htm Images present some common problems encountered during microarray production, with possible solutions. Galbraith laboratory. - [Read more] |
| Microarrays a Complete Manual PDF - http://research.nhgri.nih.gov/microarray/Protocols.pdf Microarray fabrication, coating, slide blocking, printing microarrays, RNA extraction, Sample Labeling for analysis, hybridization, complete protocol. NHGRI - [Read more] |
| Microarrays Hybridization Protocols - http://www.transcriptome.ens.fr/sgdb/protocols/hybridisation.php Excellent Photographic and VIDEO Protocols of Microarray Hybridization. Mouse microarrays hybridization protocols. Yeast microarrays hybridization protocols. Washing steps after pre-hybridization. ENS transcriptome platform website, Ile de France Genopole - [Read more] |
| Post-Processing of Arrays - http://cmgm.stanford.edu/pbrown/protocols/3_post_process.html Post-Processing of Arrays. Brown Lab. - [Read more] |
| Protocols for microarray development in use in the Vodkin Laboratory - http://soybeangenomics.cropsci.uiuc.edu/protocols/index.html Reverse transcriptase labeling with Cy3/Cy5, Staining of total DNA on slides, construction, Laser scanning, Spot quantitation, Statistics, sample tracking. Vodkin Laboratory. U.Illinois. - [Read more] |
| RNA preparation Microarray Protocol - http://www.transcriptome.ens.fr/sgdb/protocols/preparation.php RNA preparation Microarray Protocol. Yeast and Mouse Microarrays included. ENS transcriptome platform web site, Ile de France Genopole. - [Read more] |
| Sample Labeling for Microarray Analysis - http://research.nhgri.nih.gov/microarray/sample_labeling.shtml Reverse transcription labeling using Cy3 and Cy5. Sample Labeling for Microarray Analysis. NHGRI - [Read more] |
| Stripping DNA Microarray array for re-use reusing - http://www.oup.co.uk/pdf/pas/6-5-10.pdf Reusing DNA Microarrays using Stripping. Elder et al Oxford Practical Series. - [Read more] |
| Troubleshooting Arabidopsis Oligonucleotide Microarrays - http://ag.arizona.edu/microarray/Troubleshooting1.html Troubleshooting Arabidopsis Oligonucleotide Microarrays, David Galbraith. - [Read more] |
| Troubleshooting Guide to Microarray Printing - http://arrayit.com/Products/Printing/Troubleshooting_Guide/troubleshooting_guide.html Troubleshooting Guide to Microarray Printing. Arrayit. - [Read more] |
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