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Tissue Microarrays Tissue Microarrays

Tissue Microarrays an Introduction : Tissue Microarrays en Indledning:

Tissue microarrays (TMAs) are used to analyze the expression of genes simultaneously in multiple individual tissue samples on one slide. Tissue microarrays (TMAs) anvendes til at analysere udtrykket af gener samtidigt i flere individuelle vævsprøver på en slide. TMAs are composed of small 0.6 - 3.0 mm cores of tissue from donor tissue paraffin blocks which are arrayed at a high density on a slide. TMAs er sammensat af små 0,6 - 3,0 mm borekerner af væv fra donor tissue paraffinblokke, der er opstillet på en høj massefylde på en slide.

tissue microarray

Technology Insight: identification of biomarkers with tissue microarray technology Jena M Giltnane and David L Rimm Nature Clinical Practice Oncology (2004) 1, 104-111 Teknologi Indsigt: identifikation af biomarkører med tissue microarray teknologi Jena M Giltnane og David L Rimm Nature Clinical Practice Oncology (2004) 1, 104-111

Tissue Microarrays have allowed tissue traditional analysis of conventional histologic paraffin blocks to become miniturized and high-throughput. Tissue Microarrays har tilladt væv traditionel analyse af konventionelle histologic paraffinblokke at blive miniturized og høj overførselshastighed.

Histochemical and molecular detection techniques can be used on the TMA slides, and thus allow TMAs to be powerful tools to examine gene expression profiling in disease states across a variety of patients and disease conditions. Histochemical og molekylær påvisning teknikker kan bruges på TMA lysbilleder, og dermed tillade TMAs at blive stærke redskaber til at undersøge genekspression profilering i sygdommen hedder på tværs af en vifte af patienter og sygdomssymptomer.

Tissue Microarray Contents Tissue microarray Indhold

TMA Introduction TMA Indledning

Characteristics of Tissue Arrays Karakteristik af Tissue Arrays

Tissue Microarray Synthesis Tissue microarray Synthesis

Applications Ansøgninger

Advantages of TMAs Fordele ved TMAs

Types of Tissue Arrays Typer af Tissue Arrays

Analysis Software Analysis Software

Tissue Chip References Tissue Chip Referencer

Characteristics of Tissue Microarrays : Karakteristik af Tissue Microarrays:

Tissue Microarray Synthesis : Tissue microarray sammenfattende:

Tissue Microarrays are produced using a microtome which sections tissue into 4 - 5 micrometer sections (see figure 1 above). Tissue Microarrays er fremstillet ved hjælp af en microtome hvilke afsnit væv i 4 - 5 mikrometer sektioner (se figur 1 ovenfor). These core tissue biopsy sections are taken from specific areas of interest from paraffin-embedded tissue blocks.  These cynlindrical cores of tissue are re-embedded into an arrayed blank recipient blocks. Disse centrale væv biopsi afsnit er taget fra specifikke områder af interesse fra paraffin-embedded tissue blokke. Disse cynlindrical borekerner af væv er igen indlejret i en samles blank modtageren blokke. Using this method, more than 500 tissue cores can be arrayed on a single slide! Brug denne metode, mere end 500 væv borekerner kan samles på et enkelt dias! The master block was developed by Konenen et al. Føreren blokere blev udviklet af Konenen et al.

Applications of Tissue Microarrays : Ansøgninger om Tissue Microarrays:

TMA

Advantages of Tissue Arrays or Tissue Chips : Fordele ved Tissue Arrays eller væv Chips:

Types of Tissue Microarrays : Typer af Tissue Microarrays:

Cryo - Tissue Microarrays : Cryo-TMAs uses frozen tissues which are embedded in an optimal cutting temperature compound. Cryo - Tissue Microarrays: cryo-TMAs bruger frosne væv, der er indlejret i en optimal opskæring temperatur compound. These cryo-TMAs due to their freezing are superior to formalin fixed tissues for RNA and protein analysis. Disse cryo-TMAs grund af deres indefrysningen er overlegen i forhold til formalin faste væv til RNA og protein analyse. Also, antibodies work much betterl with frozen tissues. Også antistoffer arbejde langt betterl med frosne væv.

Multi-tumours Tissue Microarrays : multi-tumour TMAs have many different types of tissues aligned on the slide. Multi-tumorer Tissue Microarrays: multi-tumor TMAs har mange forskellige typer af væv bringes på dias. The technique was first used by Kononen et al., 1998. Kononen used this type of TMA to detect 6 gene amplifications. Teknikken blev første gang brugt af Kononen et al., 1998. Kononen anvendt denne type af TMA at opdage 6 genet forstærkninger. p53 expression was also examined in breast cancer tissue. p53 udtryk blev også undersøgt i brystkræft væv.

Progression Tissue Microarrays : This type of tissue microarray examines different stages of tumour (or disease) progression within a given organ. Progression Tissue Microarrays: Denne type væv microarray analyserer de forskellige stadier af tumor (eller sygdom) progression inden for et bestemt organ. For example, examination of tumours in the breast. For eksempel behandlingen af tumorer i brystet. These slides can then be assayed for markers of interest, or biochemical analysis of the samples can be done. Disse slides kan derefter skal analyseres for markører af interesse, eller biokemisk analyse af prøverne kan gøres.

Prognosis Tissue Microarrays : Disease samples such as tumour biopsies can be taken from patients and examined. Prognosis Tissue Microarrays: Sygdomme prøver såsom tumor biopsier kan være taget fra patienter og undersøgt. These samples can be used for clinical follow-ups to monitor the patient's progression. Disse prøver kan anvendes til kliniske opfølgninger til at overvåge patientens progression. Data is then analyzed and compared with other clinical data. Data er derefter analyseret og sammenlignet med andre kliniske data.

Tissue Microarray Software and Bioinformatics : Tissue microarray Software og Bioinformatik:

Software Tools for High-Throughput Analysis of Tissue Microarrays: Image Archiving and Data Analysis Softwareværktøjer til Højkapacitetsforskning Analyse af Tissue Microarrays: Image Arkivering og dataanalyse

Contains the following software: Indeholder følgende software:

TMA-Deconvoluter and Stainfinder. TMA-Deconvoluter og Stainfinder. The deconvoluter converts raw scoring worksheets from the 3-D Tissue Microarray layout into a 2-D tabulated table format suitable for further data analysis. De deconvoluter konverterer rå scoring arbejdspapirer fra 3-D Tissue microarray layout i en 2-D tabelform skemaformat egnet til videre dataanalyse.

Tissue Array Data Analysis : Software to creating an interactive database for TMA image viewing and data input. Tissue Array Data Analyse: Software til at skabe en interaktiv database for TMA billede visning og indlæsning af data.

TAD : Tissue Array Database Software for Download. TAD: Tissue Array Database Software til download.

TMAJ : set of software tools used to manage Tissue MicroArray (TMA) data and information.. TMAJ: sæt af software-værktøjer, der anvendes til at forvalte Tissue microarray (TMA) data og information ..

Original Tissue Microarray Reference Papers: Oprindelig Tissue microarray Reference Papers:

Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. Battifora H: multitumor (pølse) vævs-blok: en ny metode til immunhistokemisk antistof test. Lab Invest 1986, 55:244-248. Lab Invest 1986, 55:244-248.

Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP: Tissue microarrays for high-throughput molecular profiling of tumor specimens. Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP: Tissue microarrays for high-throughput molekylære profilering af svulst prøver. Nat Med 1998, 4:844-847. Nat Med 1998, 4:844-847.